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372
372
規格:
價格:
編號:B163719
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
372
商品貨號
B163719
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Leukemia
Applications
Spleen cells from a mouse inoculated with a B-tropic Friend murine leukemia virus (FrMuLV) were fused with NS-1 myeloma cells.
The antibody is highly specific for p15E protein from most MuLV.
Derivation
Spleen cells from a mouse inoculated with a B-tropic Friend murine leukemia virus (FrMuLV) were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against p15E envelop antigen (ENV) protein of murine leukemia viruses (MuLV)
Cellular Products
immunoglobulin; monoclonal antibody; against p15E envelop antigen (ENV) protein of murine leukemia viruses (MuLV)
Comments
Spleen cells from a mouse inoculated with a B-tropic Friend murine leukemia virus (FrMuLV) were fused with NS-1 myeloma cells.
The antibody is highly specific for p15E protein from most MuLV.
The antibody efficiently immunoprecipitates p15E and works well in Western blotting assays for p15E.
Complete Growth Medium
RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG3
Name of Depositor
B Chesebro
Deposited As
mouse (B cell); mouse (myeloma)
References

Chesebro B, et al. Characterization of mouse monoclonal antibodies specific for Friend murine leukemia virus-induced erythroleukemia cells: Friend-specific and FMR-specific antigens. Virology 112: 131-144, 1981. PubMed: 6787798

Chesebro B, et al. Characterization of monoclonal antibodies reactive with murine leukemia viruses: Use in analysis of strains of Friend MCF and Friend ecotropic murine leukemia virus. Virology 127: 134-148, 1983. PubMed: 6305011

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