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38.1
38.1
規(guī)格:
價(jià)格:
編號(hào):B163720
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
38.1
商品貨號(hào)
B163720
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with the Tp19-29 antigen associated with human T cells (CD3).
Derivation
Spleen cells were fused with NSI/1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human CD3
Cellular Products
immunoglobulin; monoclonal antibody; against human CD3
Comments
Animals were immunized with human peripheral blood T lymphocytes.
Spleen cells were fused with NSI/1 myeloma cells.
The antibody reacts with the Tp19-29 antigen associated with human T cells (CD3).
The antibody moderately inhibits alloreactive cytotoxic T lymphocyte activity and is weakly mitogenic for peripheral blood lymphocytes.
Complete Growth Medium
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
    • Subculturing
    Medium Renewal: Every 2 to 3 days
    Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 5 X 10 exp5 cells/ml.
    Isotype
    IgM
    Name of Depositor
    PJ Martin
    Deposited As
    mouse (B cell); mouse (myeloma)
    References

    . Leucocyte typing: human leucocyte differentiation antig ens detected by monoclonal antibodies. New York: Springer-Verlag; 1984.

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