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3B18

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價格:

編號:B163729

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

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產品名稱

3B18

商品貨號

B163729

Organism

Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)

Product Format

frozen

培養基

Morphology

lymphoblast

Culture Properties

mixed: adherent and suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

Carcinoma

Strain

Strain: BALB/c (B cell); BALB/c (myeloma)

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation

Spleen cells were fused with PAI myeloma cells.

Genes Expressed

immunoglobulin; monoclonal antibody; against a human mammary tumor cell cytoplasmic antigen

Cellular Products

immunoglobulin; monoclonal antibody; against a human mammary tumor cell cytoplasmic antigen

Comments

Animals were immunized with MCF7 cells (ATCC HTB-22).

Spleen cells were fused with PAI myeloma cells.

The antibody cross-reacted with some cases of fibrocystic mammary disease and one of two colon carcinomas tested.

It did not react with normal tissue nor with 17 of 18 non-mammary malignancies tested.

Complete Growth Medium

Dulbecco's modified Eagle's medium with 0.1 mM hypoxanthine, 0.02 mM thymidine, 0.01 mM 2-mercaptoethanol, and non-essential amino acids, 90%; horse serum, 10%

Subculturing

Medium Renewal: Every 2 to 3 days

Cultures can be maintained by transferring floating cells to additional flasks.

Attached cells may be subcultured by scraping.

Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.

Cryopreservation

culture medium 95%; DMSO, 5%

Culture Conditions

Temperature: 37.0°C

Isotype

IgG1

Name of Depositor

Salk Institute for Biological Studies

Deposited As

mouse (B cell); mouse (myeloma)

U.S. Patent Number

4,628,032

References

Dulbecco R, et al. Monoclonal antibody specific for a mammary tumor cytoplasmic antigen. US Patent 4,628,032 dated Dec 9 1986

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