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3C11
3C11
規格:
價格:
編號:B163731
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
3C11
商品貨號
B163731
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain:? BALB/cJ (B cell); BALB/c (myeloma)

Applications
The antibody reacts with cell wall peptidoglycans from a variety of gram- and gram+ organisms.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against bacterial cell wall peptidoglycan
Cellular Products
immunoglobulin; monoclonal antibody; against bacterial cell wall peptidoglycan
Tumorigenic
Yes
Effects
Yes, forms ascites in pristane primed BALB/cJ mice
Comments
Animals were immunized with Streptococcus mutans (BHT strain) bacterial cell wall peptidoglycan.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with cell wall peptidoglycans from a variety of gram- and gram+ organisms.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG1; kappa light chain
Name of Depositor
Temple University
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Jackson DE, et al. Monoclonal antibodies to peptidoglycan and methods of preparing same. US Patent 4,596,769 dated Jun 24 1986

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