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3C5.1
3C5.1
規格:
價格:
編號:B163732
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
3C5.1
商品貨號
B163732
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with the 220 kD catalytic subunit of DNA polymerase epsilon.
The 3C5.1 hybridoma was developed in 1993 by Gloria Chui, and can be used in Western immunoblot techniques to detect the presence of DNA polymerase epsilon.
Derivation
Animals were immunized with Mono S FPLC purified DNA polymerase epsilon.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The 3C5.1 hybridoma was developed in 1993 by Gloria Chui, and can be used in Western immunoblot techniques to detect the presence of DNA polymerase epsilon.
Genes Expressed
immunoglobulin; monoclonal antibody; against human DNA polymerase epsilon (pol epsilon)
Cellular Products
immunoglobulin; monoclonal antibody; against human DNA polymerase epsilon (pol epsilon)
Comments
Animals were immunized with Mono S FPLC purified DNA polymerase epsilon.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with the 220 kD catalytic subunit of DNA polymerase epsilon.
It does not recognize DNA polymerases alpha, beta or delta.
The 3C5.1 hybridoma was developed in 1993 by Gloria Chui, and can be used in Western immunoblot techniques to detect the presence of DNA polymerase epsilon.
Complete Growth Medium
Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM hypoxanthine and 0.008 mM thymidine, 80%; fetal bovine serum, 20%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG2a
Name of Depositor
S Linn
Deposited As
mouse (B cell); mouse (myeloma)
References

Chui G, Linn S. Further characterization of HeLa DNA polymerase epsilon. J. Biol. Chem. 270: 7799-7808, 1995. PubMed: 7536191

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