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413-15D12
413-15D12
規格:
價格:
編號:B163746
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
413-15D12
商品貨號
B163746
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized with a synthetic polypeptide with the sequence LSPEEEEKRRIRRERNKMAAAKC corresponding to peptides 132-154 of the c-fos oncogene.
The antibody reacts with the synthetic c-fos oncogene peptide.
In ELISA testing, it cross-reacts weakly with the r-fos peptide.
In immunoblot testing a protein of approximately 70 kd is detected in duck cells infected with v-fos.
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against a synthetic fos oncogene peptide
Cellular Products
immunoglobulin; monoclonal antibody; against a synthetic fos oncogene peptide
Comments
Animals were immunized with a synthetic polypeptide with the sequence LSPEEEEKRRIRRERNKMAAAKC corresponding to peptides 132-154 of the c-fos oncogene.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody reacts with the synthetic c-fos oncogene peptide. In ELISA testing, it cross-reacts weakly with the r-fos peptide.
In immunoblot testing a protein of approximately 70 kd is detected in duck cells infected with v-fos. A protein of approximately 35 kd is also detected in this cell line as well as several additional cell lines, such as ANN-1, MSTF, A431, and SSV3T3.
The IgG1 reactivity of the antibody with the c-fos peptide (132-154) was confirmed by ELISA.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.
Maintain cell density between 2 X 10 exp5 and 2 X 10 exp6 viable cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG1 and IgG2b
Name of Depositor
National Cancer Institute
Deposited As
mouse (B cell); mouse (myeloma)
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