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4A6
4A6
規格:
價格:
編號:B163758
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
4A6
商品貨號
B163758
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with an NEM sensitive fusion protein required for vesicular transport through the golgi stack.
The antibody should not be frozen, and should be used in buffers containing at least 65 mM KCl to avoid precipitation.
Derivation
Animals were immunized with purified N-ethylmaleimide (NEM) sensitive factor.
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against golgi vesicular transport protein
Cellular Products
immunoglobulin; monoclonal antibody; against golgi vesicular transport protein
Comments
Animals were immunized with purified N-ethylmaleimide (NEM) sensitive factor.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody reacts with an NEM sensitive fusion protein required for vesicular transport through the golgi stack.
The antibody should not be frozen, and should be used in buffers containing at least 65 mM KCl to avoid precipitation.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgM
Name of Depositor
JE Rothman
Deposited As
mouse (B cell); mouse (myeloma)
References

Chesebro B, et al. Characterization of monoclonal antibodies reactive with murine leukemia viruses: Use in analysis of strains of Friend MCF and Friend ecotropic murine leukemia virus. Virology 127: 134-148, 1983. PubMed: 6305011

Block MR, et al. Purification of an N-ethylmaleimide-sensitive protein catalyzing vesicular transport. Proc. Natl. Acad. Sci. USA 85: 7852-7856, 1988. PubMed: 3186695

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