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4C5G
4C5G
規格:
價格:
編號:B163762
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
4C5G
商品貨號
B163762
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with type II PtdIns 4-kinase from bovine brain.
The antibody can be used for immunoprecipitation applications and to inhibit thpe II PtdIns 4-kinase activity.
Derivation
Animals were immunized with partially purified type II phosphatidylinositol 4-kinase (PtdIns 4-kinase) from bovine brain.
Lymph node cells were fused with Sp2/0 mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against type II phosphatidylinositol 4-kinase (PtdIns 4-kinase) from bovine brain
Cellular Products
immunoglobulin; monoclonal antibody; against type II phosphatidylinositol 4-kinase (PtdIns 4-kinase) from bovine brain
Comments
Animals were immunized with partially purified type II phosphatidylinositol 4-kinase (PtdIns 4-kinase) from bovine brain.
Lymph node cells were fused with Sp2/0 mouse myeloma cells.
The antibody reacts with type II PtdIns 4-kinase from bovine brain.
The antibody causes greater than 90% inhibition of type II PtdIns 4-kinase from bovine brain, rat brain and human erythrocytes. It causes 46% inhibition of type II PtdIns 4-kinase from Xenopus laevis oocytes.
It fails to inhibit type III PtdIns 4-kinase from bovine brain or PtdIns 3-kinase from rat liver.
The antibody can be used for immunoprecipitation applications and to inhibit thpe II PtdIns 4-kinase activity.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10 exp5 viable cells/ml.
Maintain cell density between 5 X 10 exp4 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG1; kappa light chain
Name of Depositor
G Endemann
Deposited As
mouse (B cell); mouse (myeloma)
References

Endemann GC, et al. A monoclonal antibody distinguishes two types of phosphatidylinositol 4-kinase. Biochem. J. 273: 63-66, 1991. PubMed: 1846531

Del Vecchio RL, Pilch PF. Phosphatidylinositol 4-kinase is a component of glucose transporter (GLUT 4)-containing vesicles. J. Biol. Chem. 266: 13278-13283, 1991. PubMed: 1649187

De Neef RS, et al. Relationship between type II phosphatidylinositol 4-kinase activity and protein tyrosine phosphorylation in membranes from normal and sickle red cells. Eur. J. Biochem. 235: 549-556, 1996. PubMed: 8654400

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