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歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
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51.1
51.1
規格:
價格:
編號:B163775
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
51.1
商品貨號
B163775
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with an antigen associated with human cytotoxic/suppressor T cells (CD8).
The antibody strongly inhibits cytotoxic T lymphocyte function and cross-reacts with peripheral blood T cells from chimpanzee, orangutan, gibbon, baboon, macaque, capuchin monkey, squirrel monkey and spider monkey.
Derivation
Spleen cells were fused with NSI/1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human CD8
Cellular Products
immunoglobulin; monoclonal antibody; against human CD8
Comments
Animals were immunized with human peripheral blood T lymphocytes.
Spleen cells were fused with NSI/1 myeloma cells.
The antibody reacts with an antigen associated with human cytotoxic/suppressor T cells (CD8).
The antibody strongly inhibits cytotoxic T lymphocyte function and cross-reacts with peripheral blood T cells from chimpanzee, orangutan, gibbon, baboon, macaque, capuchin monkey, squirrel monkey and spider monkey.
Complete Growth Medium
Culture Medium: RPMI medium 1640 with 2 mM L-glutamine adjusted to contain
1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium
pyruvate suppelmented with 50 ?M 2-mercaptoethanol, 90%; fetal bovine serum,
10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 5 X 10 exp5 cells/ml.
Isotype
IgG2a
Name of Depositor
PJ Martin
Deposited As
mouse (B cell); mouse (myeloma)
References

Martin PJ, et al. Epitope mapping of the human surface suppressor/cytotoxic T cell molecule Tp32. J. Immunol. 132: 759-765, 1984. PubMed: 6197474

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