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514
514
規(guī)格:
價(jià)格:
編號(hào):B163776
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
514
商品貨號(hào)
B163776
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Leukemia
Applications
Animals were immunized with BB2800 leukemia cells which produce both Friend MuLV (FrMuLV) and mink cell focus inducing (MCF) FrMuLV.
The antibody neutralizes most MCF and polytropic MuLV, and reacts well with live or fixed infected cells and with Western blots.
The antibody efficiently immunoprecipitates gp70, and can be used to distinguish polytropic MuLV from ecotropic, xenotropic and amphotropic MuLV.
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against gp70 envelop antigen (ENV) protein of murine leukemia viruses (MuLV)
Cellular Products
immunoglobulin; monoclonal antibody; against gp70 envelop antigen (ENV) protein of murine leukemia viruses (MuLV)
Comments
Animals were immunized with BB2800 leukemia cells which produce both Friend MuLV (FrMuLV) and mink cell focus inducing (MCF) FrMuLV.
Spleen cells were fused with NS-1 myeloma cells.
The antibody neutralizes most MCF and polytropic MuLV, and reacts well with live or fixed infected cells and with Western blots.
The antibody efficiently immunoprecipitates gp70, and can be used to distinguish polytropic MuLV from ecotropic, xenotropic and amphotropic MuLV.
Complete Growth Medium

The base medium for this cell line is DMEM:F12 Medium (ATCC 30-2006). To make the complete growth medium, add the following components to the 500 mL of the base medium: 

  • 10% fetal bovine serum (FBS; ATCC 30-2020) 
  • 10 µg/mL insulin 
  •  5.5 µg/mL transferrin 
  •  5 ng/mL selenium 
  •  10 ng/mL EGF
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgM
Name of Depositor
B Chesebro
Deposited As
mouse (B cell); mouse (myeloma)
References

Chesebro B, et al. Characterization of monoclonal antibodies reactive with murine leukemia viruses: Use in analysis of strains of Friend MCF and Friend ecotropic murine leukemia virus. Virology 127: 134-148, 1983. PubMed: 6305011

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