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58-S

規格:

價格:

編號:B163790

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

58-S

商品貨號

B163790

Organism

Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)

Cell Type

hybridoma: B lymphocyte

培養基

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications

The antibody is type specific for HSV-1 but does not neutralize the virus.

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation

Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.

Genes Expressed

immunoglobulin; monoclonal antibody; against herpes simplex virus type 1 (HSV-1) immediate early protein ICP 4 (175000 dalton)

Cellular Products

immunoglobulin; monoclonal antibody; against herpes simplex virus type 1 (HSV-1) immediate early protein ICP 4 (175000 dalton)

Tumorigenic

Yes

Effects

Yes, forms ascites in pristane primed BALB/c mice

Comments

Animals were immunized with HSV-1.

Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.

The antibody is type specific for HSV-1 but does not neutralize the virus.

Complete Growth Medium

Culture Medium: Iscove's modified Dulbecco's medium, 90%; fetal bovine serum,
10%.

Subculturing

Medium Renewal: Every 3 to 4 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Culture Conditions

Atmosphere: 5% CO2 in air recommended

Isotype

IgG2a

Name of Depositor

The United States of America

U.S. Patent Number

4,572,896

References

Showalter SD, et al. Monoclonal antibodies to herpes simplex virus type 1 proteins, including the immediate-early protein ICP 4. Infect. Immun. 34: 684-692, 1981. PubMed: 6277788

Zweig M, et al. Monoclonal antibodies to herpes simplex virus type 1 polypeptides. US Patent 4,572,896 dated Feb 25 1986

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