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6-23 (Clone 6)

規格:

價格:

編號:B163800

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

6-23 (Clone 6)

商品貨號

B163800

Organism

Rattus norvegicus, rat

Tissue

thyroid

培養基

Cell Type

C cell

Product Format

frozen

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

medullary thyroid carcinoma

Storage Conditions

liquid nitrogen vapor phase

Genes Expressed

neurotensin; calcitonin

Comments

Secretion of neurotensin and calcitonin is stimulated by calcium, KCl, glucagon and catecholamines.

Complete Growth Medium

Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 85%; horse serum, 15%

Subculturing

Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution for 1 minute, remove trypsin EDTA, add 0.3 mL of 0.25% trypsin and let the culture sit at 37°C for 6 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 10 to 12 days.

Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:20 is recommended

Medium Renewal: 3 times per week

Cryopreservation

Freeze medium: complete growth medium, 95%; DMSO, 5%

Name of Depositor

R Gagel, FN Zeytinoglu

Deposited As

Rattus sp.

References

Zeytinoglu FN, et al. Establishment of a calcitonin-producing rat medullary thyroid carcinoma cell line. Morphological studies of the tumor and cells in culture. Endocrinology 107: 509-515, 1980. PubMed: 6248326

Zeytinoglu FN, et al. Characterization of neurotensin production by a line of rat medullary thyroid carcinoma cells. Proc. Natl. Acad. Sci. USA 77: 3741-3745, 1980. PubMed: 6932046

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