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6A7M

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編號:B163804

品牌:Mingzhoubio

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RNA

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產品名稱

6A7M

商品貨號

B163804

Organism

Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)

Cell Type

hybridoma: B lymphocyte

培養基

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain:? Biozzi High Responder (B cell); BALB/c (myeloma)

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation

Animals were immunized with type O, Rh- human erythrocytes and purified glycophorin A.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

Genes Expressed

immunoglobulin; monoclonal antibody; against glycophorin A type M

Cellular Products

immunoglobulin; monoclonal antibody; against glycophorin A type M

Tumorigenic

Yes

Effects

Yes, forms ascites in immunosuppressed BALB/c mice

Comments

Animals were immunized with type O, Rh- human erythrocytes and purified glycophorin A.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

The antibody does not react with type N glycophorin A.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.

Subculturing

Medium Renewal: 2 to 3 times per week

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Cryopreservation

culture medium 95%; DMSO, 5%

Culture Conditions

Temperature: 37.0°C

Isotype

IgG1; kappa light chain

Name of Depositor

Lawrence Livermore National Lab

Deposited As

mouse (B cell); mouse (myeloma)

U.S. Patent Number

4,767,710

(Bigbee WL, et al. Cell lines for the production of monoclonal antibodies to human glycophorin A. US Patent 4,767,710 dated Aug 30 1988)

References

Bigbee WL, et al. Monoclonal antibodies specific for the M-and N-forms of human glycophorin A. Mol. Immunol. 20: 1353-1362, 1983. PubMed: 6197636

Bigbee WL, et al. Binding specificities of eight monoclonal antibodies to human glycophorin A. Studies with McM, and MkEn(UK) variant human erythrocytes and M- and MNv-type chimpanzee erythrocytes. J. Immunol. 133: 3149-3155, 1984. PubMed: 6491283

Bigbee WL, et al. Cell lines for the production of monoclonal antibodies to human glycophorin A. US Patent 4,767,710 dated Aug 30 1988

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