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703D4
703D4
規格:
價格:
編號:B163807
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
703D4
商品貨號
B163807
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Cancer,Cancer
Strain

Strain:? BALB/c (B cell); BALB/c (myeloma)

Applications
The antibody reacts with human non-small cell lung cancer cells but not with small cell lung cancer.
The antibody also reacts with human melanomas but does not bind to a variety of other human tumors or normal human tissues.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human lung cancer
Cellular Products
immunoglobulin; monoclonal antibody; against human lung cancer
Tumorigenic
Yes
Effects
Yes, forms ascites in BALB/c mice
Comments
Animals were immunized with NCI-H157 cells (human large cell lung cancer.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with human non-small cell lung cancer cells but not with small cell lung cancer.
The antibody also reacts with human melanomas but does not bind to a variety of other human tumors or normal human tissues.
Complete Growth Medium
Modified Dulbecco's medium with 0.1 mM hypoxanthine, 40 nM aminopterin and 0.016 mM thymidine, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG2a; kappa light chain
Name of Depositor
The United States of America
U.S. Patent Number
References

Mulshine JL, et al. Monoclonal antibodies that distinguish non-small cell from small cell lung cancer. J. Immunol. 131: 497-502, 1983. PubMed: 6306102

Mulshine JL, Minna JD. Monoclonal antibodies against non small cell lung cancer. US Patent 4,569,788 dated Feb 11 1986

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