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71A7
71A7
規格:
價格:
編號:B163809
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
71A7
商品貨號
B163809
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with the Con A molecule at a site distinct from the sugar binding site.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3U1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against concanavalin A (Con A)
Cellular Products
immunoglobulin; monoclonal antibody; against concanavalin A (Con A)
Comments
Animals were immunized with C.AL-20 (a T cell line) cells that had been incubated for 48 hours with 5 mcg/ml of Con A.
Spleen cells were fused with P3U1 myeloma cells.
The antibody reacts with the Con A molecule at a site distinct from the sugar binding site.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Isotype
IgG1
Name of Depositor
FL Owen
Deposited As
mouse (B cell); mouse (myeloma)
References

Spurll GM, Owen FL. Development of a cell line secreting monoclonal antibody specific for concanavalin A and use of that antibody as an affinity immunosorbent for tissue culture media used to support long-term T cell growth. J. Immunol. Methods 39: 335-341, 1980. PubMed: 6161967

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