Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
The cells appeared to correspond to mature osteoblasts because they express alkaline phosphatase, secrete type I collagen, show a significant cyclic adenosine monophosphate response to parathyroid hormone, secrete osteocalcin, and mineralize extensively. However, in contrast to mature osteoblasts, this clone can be induced to undergo massive adipocyte differentiation, and this differentiation is accompanied by the complete loss of expression of all osteoblastic markers except alkaline phosphatase. These observations indicate that some cells that have acquired all of the characteristics of mature osteoblasts can be diverted to the adipocyte pathway. Ref
Thompson DL, et al. The derivation and characterization of stromal cell lines from the bone marrow of p53-/- mice: new insights into osteoblast and adipocyte differentiation. J. Bone Miner. Res. 13: 195-204, 1998. PubMed: 9495512
Moore EE. Preparation of immortalized cells. US Patent 5,830,682 dated Nov 3 1998
Thompson DL, et al. The derivation and characterization of stromal cell lines from the bone marrow of p53-/- mice: new insights into osteoblast and adipocyte differentiation. J. Bone Miner. Res. 13: 195-204, 1998. PubMed: 9495512
