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當(dāng)前位置: 首頁 > ATCC代理 > 83-12-5 B163838
83-12-5
83-12-5
規(guī)格:
價(jià)格:
編號:B163838
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
83-12-5
商品貨號
B163838
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with Lyt 2.2.
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse Lyt 2.2 (CD8 alpha 2.2)
Cellular Products
immunoglobulin; monoclonal antibody; against mouse Lyt 2.2 (CD8 alpha 2.2)
Comments
Animals were immunized with the BM10-5.6 cloned CTL (cytotoxic T lymphocyte) line.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody reacts with Lyt 2.2.
The antibody will fix complement.
Complete Growth Medium
Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgM
Name of Depositor
JA Bluestone
Deposited As
mouse (B cell); mouse (myeloma)
References

Leo O, et al. Identification of monoclonal antibodies specific for the T cell receptor complex by Fc receptor-mediated CTL lysis. J. Immunol. 137: 3874-3880, 1986. PubMed: 3097135

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