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86D [127.5-9]
86D [127.5-9]
規格:
價格:
編號:B163839
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
86D [127.5-9]
商品貨號
B163839
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody is specific for the sheep gamma/delta TCR and precipitates molecules of 42000 and 36000 daltons.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against the sheep (ovine) gamma/delta T cell receptor
Cellular Products
immunoglobulin; monoclonal antibody; against the sheep (ovine) gamma/delta T cell receptor
Comments
Animals were immunized with double negative (DN) sheep T cells.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is specific for the sheep gamma/delta TCR and precipitates molecules of 42000 and 36000 daltons.
Complete Growth Medium
CULTURE MEDIUM: Iscove's modified Dulbecco's medium, 90%; fetal bovine serum,
10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype
IgG1; kappa light chain
Name of Depositor
CR Mackay
Deposited As
mouse (B cell); mouse (myeloma)
References

Mackay CR, et al. Gamma/delta T cells express a unique surface molecule appearing late during thymic development. Eur. J. Immunol. 19: 1477-1483, 1989. PubMed: 2528463

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