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8A3B.6
8A3B.6
規格:
價格:
編號:B163843
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
8A3B.6
商品貨號
B163843
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody is specific for the structural protein VP7.
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against VP7 of bluetongue virus
Cellular Products
immunoglobulin; monoclonal antibody; against VP7 of bluetongue virus
Tumorigenic
Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with bluetongue virus (serotype 17).
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody is specific for the structural protein VP7.
Complete Growth Medium
Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 2 mM L-glutamine and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 6 X 10 exp4 cells/ml and maintain between 5 X 10 exp4 and 4 X 10 exp5 cells/ml.
Isotype
IgG2a
Name of Depositor
JA House
Deposited As
mouse (B cell); mouse (myeloma)
References

Appleton JA, Letchworth GJ. Monoclonal antibody analysis of serotype-restricted and unrestricted bluetongue viral antigenic determinants. Virology 124: 286-299, 1983. PubMed: 6186076

House C, et al. Detection of bluetongue group-specific antibody by competitive ELISA. J. Vet. Diagn. Invest. 2: 137-139, 1990. PubMed: 1965579

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