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8E7
8E7
規格:
價格:
編號:B163845
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
8E7
商品貨號
B163845
Organism
Homo sapiens, human
Tissue
spleen
培養基
Cell Type
B lymphoblast; Epstein-Barr virus (EBV) transforme
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
2 Cells Contain HERPESVIRUS

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody is specific for the lipopolysaccharide of the Fisher immunotype 7 of P. aeruginosa.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
B cells obtained from a patient with cystic fibrosis (known to have a chronic infection with P. aeruginosa) were transformed with Epstein-Barr virus (EBV).
Clinical Data
B cells obtained from a patient with cystic fibrosis (known to have a chronic infection with P. aeruginosa) were transformed with Epstein-Barr virus (EBV).
Genes Expressed
immunoglobulin; monoclonal antibody; against Pseudomonas aeruginosa lipopolysaccharide (LPS) of Fisher immunotype 7
Cellular Products
immunoglobulin; monoclonal antibody; against Pseudomonas aeruginosa lipopolysaccharide (LPS) of Fisher immunotype 7
Comments
B cells obtained from a patient with cystic fibrosis (known to have a chronic infection with P. aeruginosa) were transformed with Epstein-Barr virus (EBV).
The antibody is specific for the lipopolysaccharide of the Fisher immunotype 7 of P. aeruginosa.
Complete Growth Medium
Iscove's modified Dulbecco's medium with 2 mM L-glutamine, 85%; heat-inactivated fetal bovine serum, 15%
Subculturing
Medium Renewal: Every 3 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Culture Conditions
Temperature: 37.0°C
Isotype
IgM
Population Doubling Time
24 to 36 hrs
Name of Depositor
Miles Inc., Genetics Systems Corporation
U.S. Patent Number
References

Siadak AW, Lostrom ME. Human monoclonal antibodies to serotypic lipopolysaccharide determinants on Gram-negative bacteria and their production. US Patent 4,834,975 dated May 30 1989

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