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當(dāng)前位置: 首頁 > ATCC代理 > 9AE10 B163852
9AE10
9AE10
規(guī)格:
價格:
編號:B163852
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
9AE10
商品貨號
B163852
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with an antigen present on virtually all rabbit T cells and thymocytes.
It reacts with 30% of rabbit bone marrow cells, and with a majority of cells in the brain.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against rabbit T cells (T lymphocytes) and thymocytes (cytotoxic)
Cellular Products
immunoglobulin; monoclonal antibody; against rabbit T cells (T lymphocytes) and thymocytes (cytotoxic)
Tumorigenic
Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with rabbit spleen cells.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with an antigen present on virtually all rabbit T cells and thymocytes.
It reacts with 30% of rabbit bone marrow cells, and with a majority of cells in the brain.
The antigen is a glycoprotein with an apparent molecular weight of 25000 daltons and may be a rabbit analogue of the Thy-1 antigen.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
RPMI 1640 medium, 85%; horse serum, 10%; fetal bovine serum, 5%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgM
Name of Depositor
KL Knight
Deposited As
mouse (B cell); mouse (myeloma)
References

McNicholas JM, et al. Monoclonal antibodies to rabbit lymphoid cells: preparation and characterization of a T-cell-specific antibody. Mol. Immunol. 18: 815-822, 1981. PubMed: 7040945

McNicholas JM, et al. Functional studies of rabbit T lymphocytes. Immunology 43: 635-641, 1981. PubMed: 6456220

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