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9BG5
9BG5
規格:
價格:
編號:B163854
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
9BG5
商品貨號
B163854
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody neutralizes reovirus type 3 and inhibits the hemagglutinating activity of the virus.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Animals were immunized with purified reovirus type 3 virions.
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against reovirus type 3 (sigma 1 hemagglutinin)
Cellular Products
immunoglobulin; monoclonal antibody; against reovirus type 3 (sigma 1 hemagglutinin)
Comments
Animals were immunized with purified reovirus type 3 virions.
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
The antibody neutralizes reovirus type 3 and inhibits the hemagglutinating activity of the virus.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
    • Subculturing
    Medium Renewal: Every 2 to 3 days
    Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
    Isotype
    IgG2a
    Name of Depositor
    BN Fields
    Deposited As
    mouse (B cell); mouse (myeloma)
    References

    Burstin SJ, et al. Evidence for functional domains on the reovirus type 3 hemagglutinin. Virology 117: 146-155, 1982. PubMed: 6175082

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