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9D10
9D10
規格:
價格:
編號:B163855
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
9D10
商品貨號
B163855
Organism
Homo sapiens, human
Tissue
spleen
培養基
Cell Type
B lymphocyte; Epstein-Barr virus (EBV) transforme
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
2 Cells Contain HERPESVIRUS

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody is specific for the lipopolysaccharide of the Fisher immunotype 4 of P. aeruginosa.
Storage Conditions
liquid nitrogen vapor phase
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Genes Expressed
immunoglobulin; monoclonal antibody; against Pseudomonas aeruginosa lipopolysaccharide (LPS) of Fisher immunotype 4 (IATS type 1)
Cellular Products
immunoglobulin; monoclonal antibody; against Pseudomonas aeruginosa lipopolysaccharide (LPS) of Fisher immunotype 4 (IATS type 1)
Comments
B cells from peripheral blood of an individual known to have been exposed to P. aeruginosa were transformed with Epstein-Barr virus (EBV).
The antibody is specific for the lipopolysaccharide of the Fisher immunotype 4 of P. aeruginosa.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) cells/ml. Do not allow the cell concentration to reach 2 X 10(6) cells/ml.
Interval: Maintain cell density between 2 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 9,13
D16S539: 11,13
D5S818: 11,12
D7S820: 8,11
THO1: 6,7
TPOX: 8
vWA: 14,17
Isotype
IgM
Population Doubling Time
24 to 36 hrs
Name of Depositor
Miles Inc., Genetics Systems Corporation
U.S. Patent Number
References

Siadak AW, Lostrom ME. Human monoclonal antibodies to serotypic lipopolysaccharide determinants on Gram-negative bacteria and their production. US Patent 4,834,975 dated May 30 1989

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