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A11.1 M
A11.1 M
規格:
價格:
編號:B163864
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
A11.1 M
商品貨號
B163864
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized with Epstein-Barr virus transformed human B cells (HLA phenotype A11,A24,B27,B44,Bw4,Cw2).
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen and lymph node cells were fused with Sp2/08A2 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against HLA A11, A24; cytotoxic
Cellular Products
immunoglobulin; monoclonal antibody; against HLA A11, A24; cytotoxic
Comments
Animals were immunized with Epstein-Barr virus transformed human B cells (HLA phenotype A11,A24,B27,B44,Bw4,Cw2).
Spleen and lymph node cells were fused with Sp2/08A2 myeloma cells.
The antibody is cytotoxic for all HLA A11 positive cells and HLA A24 cells at an appropriate titer.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
    • Subculturing
    Medium Renewal: Every 2 to 3 days
    Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 cells/ml and maintain between 5 X 10 exp4 and 5 X 10 exp5 cells/ml.
    Culture Conditions
    Temperature: 37.0°C
    Isotype
    IgG3
    Name of Depositor
    F Grumet
    Deposited As
    mouse (B cell); mouse (myeloma)
    References

    Foung SK, et al. A monoclonal antibody against HLA-A11 and A-24. Hum. Immunol. 15: 316-319, 1986. PubMed: 2420768

    Grumet FC, et al. Monoclonal antibody (B27M2) subdividing HLA-B27. Hum. Immunol. 5: 61-72, 1982. PubMed: 6981637

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