Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease
malignant melanoma
Age
54 years
Gender
female
Storage Conditions
liquid nitrogen vapor phase
Derivation
This line was cloned by limiting dilution from the A375 melanoma cell line (see ATCC CRL-1619).
Clinical Data
female
Tumorigenic
Yes
Effects
Yes, in immunosuppressed mice
Comments
These cells will eventually die and lyse when cultured in medium containing interleukin-1 (interleukin 1, IL-1) and therefore can be used in a reliable and sensitive growth inhibition assay for IL-1.
Sensitivity to IL-1 is maximal when the cells are derived from subconfluent cultures in logarithmic growth.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, rinse with fresh 0.25% trypsin, 0.03% EDTA solution and let the culture sit at room temperature (or 37°C) until cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
