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歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
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A4.840 [4A.840]
A4.840 [4A.840]
規格:
價格:
編號:B163879
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
A4.840 [4A.840]
商品貨號
B163879
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with the adult slow isoform of human and rodent skeletal muscle myosin.
Derivation
Animals were immunized with partially purified human adult skeletal muscle myosin heavy chain.
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against adult slow myosin heavy chain (MyHC)
Cellular Products
immunoglobulin; monoclonal antibody; against adult slow myosin heavy chain (MyHC)
Comments
Animals were immunized with partially purified human adult skeletal muscle myosin heavy chain.
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
The antibody reacts with the adult slow isoform of human and rodent skeletal muscle myosin.
Complete Growth Medium
Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 0.1 mM nonessential amino acids and 1 mM sodium pyruvate, 90%; iron supplemented bovine calf serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype
IgM
Name of Depositor
HM Blau
Deposited As
mouse (B cell); mouse (myeloma)
References

Hughes SM, Blau HM. Muscle fiber pattern is independent of cell lineage in postnatal rodent development. Cell 68: 659-671, 1992. PubMed: 1531450

Condon K, et al. Development of muscle fiber types in prenatal rat hindlimb. Dev. Biol. 138: 256-274, 1990. PubMed: 2108065

Condon K, et al. Differentiation of fiber types in aneural musculature of the prenatal rat hindlimb. Dev. Biol. 138: 275-295, 1990. PubMed: 2318339

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