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A-704

規格:

價格:

編號:B163890

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

A-704

商品貨號

B163890

Organism

Homo sapiens, human

Tissue

kidney

培養基

Product Format

frozen

Morphology

epithelial

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

adenocarcinoma

Age

78 years

Gender

male

Applications

This cell line is a suitable transfection host. ref

Storage Conditions

liquid nitrogen vapor phase

Karyotype

(P59) diploid to hyperdiploid, hypertriploid to hypertetraploid with abnormalities including breaks, dicentrics and endoreduplication

Derivation

The A-704 line was derived by D.J. Giard as indicated in the description for ATCC HTB-41.

Clinical Data

male

Tumorigenic

Effects

No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation

Culture medium, 95%; DMSO, 5%

STR Profile

Amelogenin: X,Y

CSF1PO: 7,8

D13S317: 8

D16S539: 12,13

D5S818: 10,11

D7S820: 10

THO1: 7,9

TPOX: 11

vWA: 14,18

Isoenzymes

AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 2

Me-2, 1

PGM1, 1

PGM3, 1-2

Name of Depositor

DJ Giard

Deposited As

Homo sapiens

References

Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758

Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047

Cross References

Nucleotide (GenBank) : U02327 Human clone ndf19 neu differentiation factor mRNA, partial cds.

Nucleotide (GenBank) : U02330 Human clone ndf33 neu differentiation factor mRNA, partial cds.

Nucleotide (GenBank) : U02326 Human clone ndf43 neu differentiation factor mRNA, complete cds.

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