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A717
A717
規(guī)格:
價格:
編號:B163891
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
A717
商品貨號
B163891
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody is specific for glycated human serum albumin.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against glycated serum albumin (glycoalbumin)
Cellular Products
immunoglobulin; monoclonal antibody; against glycated serum albumin (glycoalbumin)
Tumorigenic
Yes
Effects
Yes, forms ascites in pristane primed BALB/c mice
Comments
Animals were immunized with native glycated human serum albumin.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is specific for glycated human serum albumin.
It does not react with unglycated albumin.
Complete Growth Medium
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
    • Subculturing
    Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 1 X 10 exp6 cells/ml.
    Medium Renewal: Every 2 to 3 days
    Culture Conditions
    Temperature: 37.0°C
    Isotype
    IgG , IgG
    Name of Depositor
    Exocell, Inc.
    Deposited As
    mouse (B cell); mouse (myeloma)
    U.S. Patent Number
    References

    Cohen MP. Treatment of complications of diabetes with substances reactive with the fructosyl-lysine structure in glycated albumin. US Patent 5,518,720 dated May 21 1996

    Cohen MP, Hud E. Production and characterization of monoclonal antibodies against human glycoalbumin. J. Immunol. Methods 117: 121-129, 1989. PubMed: 2913157

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