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AHH-1

規(guī)格:

價(jià)格:

編號(hào):B163921

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

AHH-1

商品貨號(hào)

B163921

Organism

Homo sapiens, human

Tissue

peripheral blood

培養(yǎng)基

Cell Type

B lymphocyte

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

2 [Cells contain Herpesvirus]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age

33 years

Gender

male

Ethnicity

Caucasian

Applications

The cells have a high level of oxidative activity, and and can be used in mutagenesis assays for substances that are metabolized into mutagenic products.

They are are sensitive to killing by benzo[a]pyrene.

Storage Conditions

liquid nitrogen vapor phase

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Karyotype

near diploid

Images

Clinical Data

male

Caucasian

33 years

Comments

The cells have a high level of oxidative activity, and and can be used in mutagenesis assays for substances that are metabolized into mutagenic products. They are are sensitive to killing by benzo[a]pyrene. The cells carry an endogenous Epstein-Barr virus (EBV) genome and are EBNA positive.

Complete Growth Medium

RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; horse serum, 10%

Subculturing

Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.

Medium Renewal: 2 to 3 times per week

Note: The cells grow as floating aggregates of large cell clusters.

Cryopreservation

Freeze medium: culture medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

STR Profile

Amelogenin: X,Y

CSF1PO: 10

D13S317: 11,13

D16S539: 10

D5S818: 12,13

D7S820: 10,11

THO1: 6,9.3

TPOX: 8,9

vWA: 18,19

Population Doubling Time

16 to 19 hrs

Name of Depositor

Massachusetts Institute of Technology

Deposited As

Homo sapiens

U.S. Patent Number

4,532,204

References

Crespi CL, Thilly WG. Mutation assays involving blood cells that metabolize toxic substances. US Patent 4,532,204 dated Jul 30 1985

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