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歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
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alpha BL5C2.870009 [870009, aBL5C2.870009]
alpha BL5C2.870009 [870009, aBL5C2.870009]
規格:
價格:
編號:B163928
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
alpha BL5C2.870009 [870009, aBL5C2.870009]
商品貨號
B163928
Organism
bovine (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody neutralizes BHV-1 and is protective against BHV-1 induced mortality in rabbits.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Lymph node cells were fused with Sp2/0 mouse myeloma cells.
Initial hybrids were refused with additional lymph node cells from the same animal and reselected.
Genes Expressed
immunoglobulin; monoclonal antibody; against bovine herpesvirus 1 (BHV-1)
Cellular Products
immunoglobulin; monoclonal antibody; against bovine herpesvirus 1 (BHV-1)
Comments
Animals were immunized with live BHV-1.
Lymph node cells were fused with Sp2/0 mouse myeloma cells.
Initial hybrids were refused with additional lymph node cells from the same animal and reselected.
The antibody neutralizes BHV-1 and is protective against BHV-1 induced mortality in rabbits.
Complete Growth Medium
Culture Medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%;
horse serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
fetal bovine serum, 85%; DMSO, 15%
Isotype
IgG1
Name of Depositor
The United States of America
U.S. Patent Number
References

Levings RL, Stoll IR. Bovine monoclonal antibodies to bovine herpesvirus I from sequential fusion heterohybridomas. US Patent 5,026,646 dated Jun 25 1991

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