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anti-130-kDa Mesothelial-Ciliated Cells
anti-130-kDa Mesothelial-Ciliated Cells
規格:
價格:
編號:B163944
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
anti-130-kDa Mesothelial-Ciliated Cells
商品貨號
B163944
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The hybridoma cell line anti-130-kDa Mesothelial-Ciliated Cells produces an antibody (IgM; kappa light chain)) that is specific for the 130-kDa mesothelial and ciliated cell protein in human and rat lungs.
In formalin-fixed, paraffin-embedded tissues from adult human and rat organs, the antibody specifically stained the luminal/apical surfaces of pulmonary and nonpulmonary ciliated and mesothelial cells.
The antibody may be useful in the diagnosis of epithelial mesotheliomas and ovarian/endometrial carcinomas, because it selectively stains these tumors and is reactive with formalin-fixed, paraffin-embedded tissues.
Storage Conditions
liquid nitrogen vapor phase
Derivation
The surfactant was isolated by lung lavage from cadaveric lungs and processed to yield surfactant binding proteins.
Genes Expressed
immunoglobulin; monoclonal antibody; against the 130-kDa mesothelial and ciliated cell protein
Cellular Products
immunoglobulin; monoclonal antibody; against the 130-kDa mesothelial and ciliated cell protein
Comments
The hybridoma cell line anti-130-kDa Mesothelial-Ciliated Cells produces an antibody (IgM; kappa light chain)) that is specific for the 130-kDa mesothelial and ciliated cell protein in human and rat lungs.
By Western blotting, the antibody stained a protein of about 130 kDa.
In formalin-fixed, paraffin-embedded tissues from adult human and rat organs, the antibody specifically stained the luminal/apical surfaces of pulmonary and nonpulmonary ciliated and mesothelial cells.
Airway cell staining was detectable in human fetal lungs at 12 weeks of gestation and at Day 18 of gestation in fetal rat.
The antibody reacted with human and rat fetal mesothelial cells at the gestational ages of 15 weeks and 17 days, respectively. It also stained ciliated cells in endosalpinx and endometrium.
The antibody may be useful in the diagnosis of epithelial mesotheliomas and ovarian/endometrial carcinomas, because it selectively stains these tumors and is reactive with formalin-fixed, paraffin-embedded tissues.
The cell line was formed by the fusion of P3/X63/Ag mouse myeloma cells with splenocytes from a BALB/c mouse inoculated with surfactant binding proteins.
The surfactant was isolated by lung lavage from cadaveric lungs and processed to yield surfactant binding proteins.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 x 105 viable cells/mL. Maintain cell density between 1 x 105 and 1 x 106  viable cells/mL. Add fresh medium as cell density increases.

Medium Renewal: Every 2 to 3 days

Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype
IgM, kappa light chain
Name of Depositor
G Singh
Deposited As
Mus musculus (B cell); Mus musculus (myeloma)
References

Singh G, et al. Expression of a 130-kDa mesothelial and ciliated cell Ag (MCp130) in normal and developing human and rat lung and its role as a diagnostic marker for mesotheliomas and tumors of the female reproductive system. Lab. Invest. 73: 48-58, 1995. PubMed: 7603040

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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