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當(dāng)前位置: 首頁(yè) > ATCC代理 > Anti-My-10 clone 28/8/8/14/4 B163946
Anti-My-10 clone 28/8/8/14/4
Anti-My-10 clone 28/8/8/14/4
規(guī)格:
價(jià)格:
編號(hào):B163946
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Anti-My-10 clone 28/8/8/14/4
商品貨號(hào)
B163946
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody binds to KG-1a cells and to 5 to 10% of human marrow cells (blast cells).
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against an antigen on myeloid cells
Cellular Products
immunoglobulin; monoclonal antibody; against an antigen on myeloid cells
Comments
Animals were immunized with KG-1a cells (see ATCC CCL-246.1).
Spleen cells were fused with myeloma cells.
The antibody binds to KG-1a cells and to 5 to 10% of human marrow cells (blast cells).
Complete Growth Medium
RPMI 1640 medium with non-essential amino acids, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium, 90%; DMSO, 10%
Isotype
IgG1; kappa light chain
Name of Depositor
Johns Hopkins School of Medicine
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Civin CI. Human stem cells. US Patent 4,714,680 dated Dec 22 1987

Civin CI, et al. My-1, new myeloid-specific antigen identified by a mouse monoclonal antibody. Blood 57: 842-845, 1981. PubMed: 6163493

Page PL, et al. Cytotoxic reduction and regeneration of murine marrow stem cells. Exp. Hematol. 11: 202-211, 1983. PubMed: 6832246

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