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B21-2
B21-2
規格:
價格:
編號:B163977
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
B21-2
商品貨號
B163977
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody immunoprecipitates polypeptides typical of class II antigens, and competes with alloantisera against Ia-8 (anti I-A b,d) but not Ia-7 (anti I-E d,k) antisera.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3X63Ag8-U1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against I-A b,d
Cellular Products
immunoglobulin; monoclonal antibody; against I-A b,d
Tumorigenic
Yes
Effects
Yes, in irradiated (600R) pristane primed BALB/c mice
Comments
Animals were immunized intravenously with live dendritic cells from BALB/c mice.
Spleen cells were fused with P3X63Ag8-U1 myeloma cells.
The antibody immunoprecipitates polypeptides typical of class II antigens, and competes with alloantisera against Ia-8 (anti I-A b,d) but not Ia-7 (anti I-E d,k) antisera.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG2b
Name of Depositor
RM Steinman
Deposited As
rat (B cell); mouse (myeloma)
References

Steinman RM, et al. Lymphokine enhances the expression and synthesis of Ia antigens on cultured mouse peritoneal macrophages. J. Exp. Med. 152: 1248-1261, 1980. PubMed: 6448907

Nussenzweig MC, et al. Studies of the cell surface of mouse dendritic cells and other leukocytes. J. Exp. Med. 154: 168-187, 1981. PubMed: 7252426

Schuler G, Steinman RM. Murine epidermal Langerhans cells mature into potent immunostimulatory dendritic cells in vitro. J. Exp. Med. 161: 526-546, 1985. PubMed: 3871837

Wong P, Rudensky AY. Phenotype and function of CD4+ T cells in mice lacking invariant chain. J. Immunol. 156: 2133-2142, 1996. PubMed: 8690902

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