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B27M2
B27M2
規格:
價格:
編號:B163979
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
B27M2
商品貨號
B163979
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized with human peripheral blood leukocytes (HLA phenotype A2,A28,B27,B44,Bw4,Cw2).
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen and lymph node cells were fused with Sp2/08A2 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against HLA B27, Bw47; cytotoxic
Cellular Products
immunoglobulin; monoclonal antibody; against HLA B27, Bw47; cytotoxic
Comments
Animals were immunized with human peripheral blood leukocytes (HLA phenotype A2,A28,B27,B44,Bw4,Cw2).
Spleen and lymph node cells were fused with Sp2/08A2 myeloma cells.
The antibody is cytotoxic for a subpopulation of HLA B27 positive cells and for all HLA Bw47 positive cells at an appropriate titer.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Culture Medium: Modified RPMI 1640 medium (see below) with 50 uM
2-mercaptoethanol, 90%; fetal bovine serum, 10%.
RPMI 1640 100 mL
L-Glutamine (100x) 1 mL
Solution FM 1 mL
Non-essential amino acids (100x) 1 mL
Fetal Bovine Serum 12 mL
Solution FM (100x)
1. 1320 mg oxalacetic acid (100 mM, MW 132).
2. 100 mg crystalline bovine insulin (20 units/mL, 25 units/mg).
Add 1 and 2, stir at 37°C. Add Na pyruvate 450 mg (41 mM, FW 110). Bring up to
100 mL with distilled water. Stir at 37°C until solution dissolves. Filter,
aliquot and store frozen.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgM
Name of Depositor
F Grumet
Deposited As
mouse (B cell); mouse (myeloma)
References

Grumet FC, et al. Monoclonal antibody (B27M2) subdividing HLA-B27. Hum. Immunol. 5: 61-72, 1982. PubMed: 6981637

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