Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
The cells are deficient in lysyl oxidase.
ATCC CCL-197 is an age, sex and strain matched control for this line.
Tested and found negative for ectromelia virus (mousepox).
Subcultivation Ratio: 1:2 to 1:5
Medium Renewal: Every 2 to 3 days
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells: A Manual of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley - Liss, N.Y., 2005.
Complete culture medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC® Catalog No. 4-X.
Temperature: 37°C
Atmosphere: Air, 95%; CO2, 5%
Starcher BC, et al. Lysyl oxidase deficiency in lung and fibroblasts from mice with hereditary emphysema. Biochem. Biophys. Res. Commun. 78: 706-712, 1977. PubMed: 20889
Starcher B, et al. Abnormal cellular copper metabolism in the blotchy mouse. J. Nutr. 108: 1229-1233, 1978. PubMed: 27591
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
