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BTV10XSp2/0-Ag14-10D4.90
BTV10XSp2/0-Ag14-10D4.90
規格:
價格:
編號:B164054
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
BTV10XSp2/0-Ag14-10D4.90
商品貨號
B164054
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Tissue
spleen
培養基
Cell Type
hybridoma:lymphoblast B lymphocyte
Product Format
frozen
Morphology
lymphoblast
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age
adult
Strain

Strain:? BALB/c (B cell); BALB/c (myeloma)

Applications
The antibody reacts with a viral protein associated with the replication BTV.
It can be used in immunofluorescence assays to identify the U.S. serotypes of BTV, and differentiate between BTV and the closely related virus, epizootic hemorrhagic disease virus (EHDV).
Storage Conditions
liquid nitrogen vapor phase
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Bluetongue virus (BTV, orbivirus)
Cellular Products
immunoglobulin; monoclonal antibody; against Bluetongue virus (BTV, orbivirus)
Tumorigenic
Yes
Effects
Yes, forms ascites in pristane primed BALB/c mice
Comments
Animals were immunized with serotype 10 BTV.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with a viral protein associated with the replication BTV.
It can be used in immunofluorescence assays to identify the U.S. serotypes of BTV, and differentiate between BTV and the closely related virus, epizootic hemorrhagic disease virus (EHDV).
The immunofluorescence pattern is characterized as abundant discrete granules and large and small spherical inclusions in the cytoplasm of infected cells.
Complete Growth Medium
Culture medium: HB101, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Isotype
IgG2a kappa
Name of Depositor
The United States of America
U.S. Patent Number
References

Jochim MM, Jones SC. Monoclonal antibodies to bluetongue virus antigen. US Patent 4,873,189 dated Oct 10 1989

. . Proc. Am. Assoc. Vet. Lab. Diag. : 277-283, 1983.

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