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歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
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BW5147(T200-a)5.2
BW5147(T200-a)5.2
規(guī)格:
價格:
編號:B164058
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
BW5147(T200-a)5.2
商品貨號
B164058
Organism
Mus musculus, mouse
Cell Type
T lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
lymphoma
Strain
AKR/J
Applications
They are also negative for the CD45, T200, and Lea antigens.
The cells are resistant to 0.1 mM 6-thioguanine and 1 mM ouabain.
Storage Conditions
liquid nitrogen vapor phase
Derivation
This line is a derivative of BW5147.G.1.4.OUAR.1 (ATCC CRL-1588).
Comments
This line is a derivative of BW5147.G.1.4.OUAR.1 (ATCC CRL-1588).
The cells do not synthesize detectable amounts of the CD45 glycoprotein and do not express this molecule on the cell surface.
They are also negative for the CD45, T200, and Lea antigens.
The cells are resistant to 0.1 mM 6-thioguanine and 1 mM ouabain.
Complete Growth Medium
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; heat-inactivated horse serum, 10%
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 to 8 X 10(4) viable cells/ml. Maintain cell density between 5 X 10(4) and 1 X 10(6) viable cells/ml. Do not exceed 5 X 10(5) cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Name of Depositor
R Hyman
Deposited As
Mus musculus
References

Hyman R, Trowbridge I. Two complementation classes of T200 (Ly-5) glycoprotein-negative mutants. Immunogenetics 12: 511-523, 1981. PubMed: 7194313

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