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C1498
C1498
規格:
價格:
編號:B164071
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
C1498
商品貨號
B164071
Organism
Mus musculus, mouse
Cell Type
lymphoblast
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
acute myeloid leukemia
Gender
female
Strain
C57BL/6J
Applications
Tested and found negative for ectromelia virus (mousepox).
They are resistant to 0.1 mM cortisol.
Storage Conditions
liquid nitrogen vapor phase
Clinical Data
female
Antigen Expression
Antigen expression: The cells do not express B7-1.
Comments
They are resistant to 0.1 mM cortisol.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can beestablished by centrifugation with subsequent resuspension in fresh medium at 1 to 3 x 105 viable cells/mL. A saturation density of 1 to 2 X 106 is obtainable.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Name of Depositor
P Ralph
Deposited As
Mus musculus
References

Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304

Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714

Boyer MW, et al. Thr role of B7 costimulation by murine acute myeloid leukemia in the generation and function of a CD8+ T-cell line with potent in vivo graft-versus-leukemia properties. Blood 89: 3477-3485, 1997. PubMed: 9129056

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