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C171 [Clone 171]
C171 [Clone 171]
規格:
價格:
編號:B164074
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
C171 [Clone 171]
商品貨號
B164074
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain:? BALB/c (B cell); BALB/c (myeloma)

Applications
The antibody reacts equivalently with all 3 isoforms of KTI and binds equally well to KTI - trypsin complexes.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Kunitz soybean trypsin inhibitor
Cellular Products
immunoglobulin; monoclonal antibody; against Kunitz soybean trypsin inhibitor
Tumorigenic
Yes
Effects
Yes, forms ascites in BALB/c mice
Comments
Animals were immunized with Kunitz soybean trypsin inhibitor (KTI).
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts equivalently with all 3 isoforms of KTI and binds equally well to KTI - trypsin complexes.
It does not discriminate between native and denatured KTI.
Complete Growth Medium
Culture medium: RPMI 1640 with 2 mM L-glutamine, 1 mM sodium pyruvate and
10 mM HEPES, 85%; horse serum, 15%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG1; kappa light chain
Name of Depositor
The United States of America
U.S. Patent Number
References

Brandon DL, et al. Monoclonal antibodies to soybean Kunitz trypsin inhibitor and immunoassay methods. US Patent 4,959,310 dated Sep 25 1990

Brandon DL, et alAntigenicity of soybean protease inhibitorsIn: Brandon DL, et alProtease inhibitors as cancer chemopreventive agentsNew YorkPlenum Presspp. 107-129, 1993

. . J. Food Sci. 53: 97-101, 1988.

J. Agric. Food Chem. 36: 1336-1341, 1988.

. . Crop Sci. 32: 1502-1505, 1992.

J. Agric. Food Chem. 39: 327-335, 1991.

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