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C1R-neo

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編號:B164077

品牌:Mingzhoubio

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產品名稱

C1R-neo

商品貨號

B164077

Organism

Homo sapiens, human

Cell Type

B lymphoblast; Epstein-Barr virus (EBV) transforme

培養基

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

2 Cells contain herpesvirus

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications

C1R-neo is a stable transfectant cell line established in 1987 by transfection by electroporation of the C1R cell line with a modified neomycin drug-resistant eukaryotic vector, pSP65-Neo.

C1R-neo does not secrete or express HLA B7.

B-LCL by gamma irradiation followed by selection for Class I monoclonal antibodies and complement.

C1R is a human B-cell lymphoblastoid line lacking surface HLA A and B antigens.

C1R was derived from Hmy.

C1R-neo may be used as a control for C1R-sB7 (CRL-2370), in CTL experiments and for producing control supernatants.

Derivation

C1R-neo is a stable transfectant cell line established in 1987 by transfection by electroporation of the C1R cell line with a modified neomycin drug-resistant eukaryotic vector, pSP65-Neo. The vector did not carry an insert.

C1R is a human B-cell lymphoblastoid line lacking surface HLA A and B antigens. C1R was derived from Hmy.2 B-LCL by gamma irradiation followed by selection for Class I monoclonal antibodies and complement.

Comments

C1R-neo does not secrete or express HLA B7.

C1R-neo may be used as a control for C1R-sB7 (CRL-2370), in CTL experiments and for producing control supernatants.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 X 10(5) viable cells/ml.

Maintain cultures at cell concentrations between 5 X 10(5) and 2 X 10(6) viable cells/ml.

Cryopreservation

culture medium 95%; DMSO, 5%

Culture Conditions

Temperature: 37.0°C

STR Profile

Amelogenin: X

CSF1PO: 6,10

D13S317: 11,13

D16S539: 9,13

D5S818: 10,13

D7S820: 7,12

THO1: 8

TPOX: 8

vWA: 17

Name of Depositor

F Grumet

Year of Origin

1987

References

Storkus WJ, et al. Reversal of natural killing susceptibility in target cells expressing transfected class I HLA genes. Proc. Natl. Acad. Sci. USA 86: 2361-2364, 1989. PubMed: 2784569

Grumet FC, et al. Soluble form of an HLA-B7 class I antigen specifically suppresses humoral alloimmunization. Hum. Immunol. 40: 228-234, 1994. PubMed: 7960967

Hiraki DD, et al. Bioengineered soluble HLA-B7. Genesis, characterization, and occurrence of dimerization. Hum. Immunol. 40: 235-246, 1994. PubMed: 7960968

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