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Capan-2
Capan-2
規(guī)格:
價格:
編號:B164120
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Capan-2
商品貨號
B164120
Organism
Homo sapiens, human
Tissue
pancreas
培養(yǎng)基
Product Format
frozen
Morphology
polygonal
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
adenocarcinoma
Age
56 years
Gender
male
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Karyotype
modal number = 69; range = 65 to 74.
This is a pseudotriploid human cell line with the modal chromosome number of 69, occurring in 24% of cells. However, cells having chromosome counts from 65 to 70 also occurred at high frequencies. The rate of cells with higher ploidies was 7%. There were eight marker chromosomes common to most cells. They included paired del(1) (p22), t(8q11q), i(14q), single i(7p) and four others. Two more markers were seen only in some cells. Only one normal X chromosome was found in G-banded preparation. The Y chromosome was not detected in the Q-banded preparation.
Images
Cell Micrograph of ATCC HTB-80, Capan-2
Clinical Data
56 years
Caucasian
male
Antigen Expression
Antigen expression: Blood Type B; Rh+
Genes Expressed

Blood Type B; Rh+, 

The cells produce high levels of MUC-1 mucin mRNA, low levels of MUC-2 mRNA but do not express the MUC-3 gene.

Cellular Products
mucin (apomucin, MUC-1, MUC-2)
Tumorigenic
Yes
Effects
Yes, in nude mice; forms well differentiated adenocarcinoma consistent with pancreatic carcinoma
Complete Growth Medium
The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 9,13
D5S818: 11,12
D7S820: 9,11
THO1: 9.3
TPOX: 8
vWA: 17
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 2
PGM1, 1
PGM3, 2
Name of Depositor
J Fogh
Deposited As
Homo sapiens
Year of Origin
1975
References

Dahiya R, et al. Mucin synthesis and secretion in various human epithelial cancer cell lines that express the MUC-1 mucin gene. Cancer Res. 53: 1437-1443, 1993. PubMed: 8443822

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