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CC17
CC17
規格:
價格:
編號:B164127
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
CC17
商品貨號
B164127
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody is specific for an antigen on bovine T cells that is analogous to the human CD5 antigen.
CC17 reacts with CD5.1 allele.
Storage Conditions
liquid nitrogen vapor phase
Derivation
Spleen cells were fused with NS1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against bovine CD5 (BoCD5)
Cellular Products
immunoglobulin; monoclonal antibody; against bovine CD5 (BoCD5)
Comments
Animals were immunized with neonatal bovine thymocytes.
Spleen cells were fused with NS1 myeloma cells.
The antibody is specific for an antigen on bovine T cells that is analogous to the human CD5 antigen.
The antigen is expressed on CD2 positive T cells, WC1 positive T cells and a subset of B cells.
CC17 reacts with CD5.1 allele.
This antibody was included in the panel of the International Workshop on Bovine, Sheep and Goat Leukocyte Differentiation Antigens in 1989 and 1992.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(5) cells/ml.
Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype
IgG1
Name of Depositor
CJ Howard
Deposited As
mouse (B cell); mouse (myeloma)
References

Howard CJ, et al. Demonstration of two allelic forms of the bovine T cell antigen Bo5 (CD5) and studies of their inheritance. Anim. Genet. 20: 351-359, 1989. PubMed: 2515774

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