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CC21
CC21
規(guī)格:
價格:
編號:B164129
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
CC21
商品貨號
B164129
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody is specific for a 145000 dalton antigen on bovine B cells.
Derivation
Spleen cells were fused with NS1 myeloma cells.
Genes Expressed
The antibody reacts with rat type 1 myosin heavy chains, and binds to slow type I fibres in rat muscle.
immunoglobulin; monoclonal antibody; against WC3 bovine B cell antigen (BoWC3),Expression of WC3 correlates with IgM expression on circulating B cells.
Cellular Products
immunoglobulin; monoclonal antibody; against WC3 bovine B cell antigen (BoWC3)
Comments
Animals were immunized with bovine alveolar macrophages.
Spleen cells were fused with NS1 myeloma cells.
The antibody is specific for a 145000 dalton antigen on bovine B cells.
Expression of WC3 correlates with IgM expression on circulating B cells.
The antigen is also expressed on dendritic cells in lymphoid tissues.
It appears to be the analogue to human CD21.
This antibody was included in the panel of the International Workshop on Bovine, Sheep and Goat Leukocyte Differentiation Antigens in 1989.
Complete Growth Medium
Culture Medium: RPMI medium 1640, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype
IgG1
Name of Depositor
CJ Howard
Deposited As
mouse (B cell); mouse (myeloma)
References

Naessens J, et al. Characterization of a bovine leucocyte differentiation antigen of 145,000 MW restricted to B lymphocytes. Immunology 69: 525-530, 1990. PubMed: 2185984

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