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CC29
CC29
規格:
價格:
編號:B164130
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
CC29
商品貨號
B164130
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody is specific for an antigen on bovine T cells that is analogous to the human CD5 antigen.
Derivation
Spleen cells were fused with NS1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against bovine CD5 (BoCD5)
Cellular Products
immunoglobulin; monoclonal antibody; against bovine CD5 (BoCD5)
Comments
Animals were immunized with neonatal bovine thymocytes.
Spleen cells were fused with NS1 myeloma cells.
The antibody is specific for an antigen on bovine T cells that is analogous to the human CD5 antigen.
The antigen is expressed on CD2 positive T cells, WC1 positive T cells and a subset of B cells.
This antibody was included in the panel of the International Workshop on Bovine, Sheep and Goat Leukocyte Differentiation Antigens in 1989.
Complete Growth Medium
Culture Medium: RPMI medium 1640, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype
IgG1
Name of Depositor
CJ Howard
Deposited As
mouse (B cell); mouse (myeloma)
References

Howard CJ, et al. In vivo depletion of BoT4 (CD4) and of non-T4/T8 lymphocyte subsets in cattle with monoclonal antibodies. Eur. J. Immunol. 19: 757-764, 1989. PubMed: 2567247

Howard CJ, et al. Two monoclonal antibodies (CC17, CC29) recognizing an antigen (Bo5) on bovine T lymphocytes, analogous to human CD5. Vet. Immunol. Immunopathol. 19: 127-139, 1988. PubMed: 3055661

Oldham G, Howard CJ. Suppression of bovine lymphocyte responses to mitogens following in vivo and in vitro treatment with dexamethasone. Vet. Immunol. Immunopathol. 30: 161-177, 1992. PubMed: 1534431

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