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CC58
CC58
規(guī)格:
價(jià)格:
編號(hào):B164139
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
CC58
商品貨號(hào)
B164139
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody is specific for bovine CD8 T lymphocyte antigen.
Storage Conditions
liquid nitrogen vapor phase
Derivation
Spleen cells were fused with NS1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against bovine CD8 (BoCD8)
Cellular Products
immunoglobulin; monoclonal antibody; against bovine CD8 (BoCD8)
Comments
Animals were immunized with bovine lymphocytes and thymocytes.
Spleen cells were fused with NS1 myeloma cells.
The antibody is specific for bovine CD8 T lymphocyte antigen.
It appears to be reactive only with the alpha/beta heterodimer and not the alpha/alpha homodimer.
This antibody was included in the panel of the International Workshop on Bovine, Sheep and Goat Leukocyte Differentiation Antigens in 1989.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(5) cells/ml.
Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype
IgG1
Name of Depositor
CJ Howard
Deposited As
mouse (B cell); mouse (myeloma)
References

Hein WR, et al. Epitopes of the T19 lymphocyte surface antigen are extensively conserved in ruminants. Vet. Immunol. Immunopathol. 27: 173-181, 1991. PubMed: 1708546

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