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CCD-1132Sk
CCD-1132Sk
規(guī)格:
價格:
編號:B164188
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
CCD-1132Sk
商品貨號
B164188
Organism
Homo sapiens, human
Tissue
skin
培養(yǎng)基
Cell Type
fibroblast
Product Format
frozen
Morphology
fibroblast
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
normal
Age
18 years
Gender
female
Ethnicity
Black
Applications
The cell line was established from skin taken from the breast of a female with macromastia.
Derivation
The cell line was established from skin taken from the breast of a female with macromastia.
Clinical Data
The cell line was established from skin taken from the breast of a female with macromastia.
female
Black
18 years
Comments
The cell line was established from skin taken from the breast of a female with macromastia.
Cells senesce after approximately 56 population doublings.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor
F.Haq, ATCC
Deposited As
human
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