Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age
9 years
Gender
male
Ethnicity
Asian
Applications
The line was derived from normal tissue from patient who died following trauma to the head.
Karyotype
normal human male; diploid; stable
Derivation
The line was derived from normal tissue from patient who died following trauma to the head.
Clinical Data
The line was derived from normal tissue from patient who died following trauma to the head.
male
Asian
9 years
Antigen Expression
blood type O; Rh +
Genes Expressed
blood type O; Rh +
Comments
The line was derived from normal tissue from patient who died following trauma to the head.
The cells are capable of at least 12 population doublings beyond the current freeze.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin for several minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 5 to 7 days.
