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CDR2
CDR2
規格:
價格:
編號:B164222
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
CDR2
商品貨號
B164222
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The primary specificity is for the thymic cortex, although there is faint non-epithelial reactivity in the medulla.
It cross-reacts with epithelial elements in human kidney, tonsils, pancreatic ducts, lymph nodes and esophagus.
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human cortical thymic epithelium
Cellular Products
immunoglobulin; monoclonal antibody; against human cortical thymic epithelium
Comments
Animals were immunized with homogenized human thymus (1.5 to 2 years of age).
Spleen cells were fused with Sp2/0 myeloma cells.
The primary specificity is for the thymic cortex, although there is faint non-epithelial reactivity in the medulla.
The antibody does not react with mouse or rat tissues.
It cross-reacts with epithelial elements in human kidney, tonsils, pancreatic ducts, lymph nodes and esophagus.
Complete Growth Medium

Iscove's Modified Dulbecco's Medium (ATCC® 30-2005), 90%; fetal bovine serum (ATCC® 30-2020), 10%.

Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG2b
Name of Depositor
RV Rouse
Deposited As
mouse (B cell); mouse (myeloma)
References

Rouse RV, et al. Monoclonal antibodies reactive with subsets of mouse and human thymic epithelial cells. J. Histochem. Cytochem. 36: 1511-1517, 1988. PubMed: 2461413

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