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CH26-1352
CH26-1352
規格:
價格:
編號:B164243
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
CH26-1352
商品貨號
B164243
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with FO myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against double stranded DNA
Cellular Products
immunoglobulin; monoclonal antibody; against double stranded DNA
Tumorigenic
Yes
Effects
Yes, forms ascites in pristane primed BALB/c mice
Comments
Animals were immunized with synthetic polynucleotide homopolymers [poly(dA-dT)-poly(dA-dT) and poly(dI-dC)-poly(dI-dC)] then boosted with foreign native DNA (EcoR1 digested pBR322 DNA).
Spleen cells were fused with FO myeloma cells.
Complete Growth Medium
Culture Medium: Iscove's modified Dulbecco's medium, 90%; fetal bovine serum,
10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 90%; DMSO, 10%
Isotype
IgM
Name of Depositor
Cetus Corp.
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Huang CM, Cohen SN. Monoclonal antibody having specificity for the double-stranded conformation of native DNA and diagnostic methods using same. US Patent 4,623,627 dated Nov 18 1986

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