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CH-EB6
CH-EB6
規格:
價格:
編號:B164246
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
CH-EB6
商品貨號
B164246
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with both subclasses (IgA1 and IgA2).
Tested and found negative for ectromelia virus (mousepox).
Derivation
Animals were immunized with purified IgA1,k.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human IgA (IgA1, IgA2)
Cellular Products
immunoglobulin; monoclonal antibody; against human IgA (IgA1, IgA2)
Comments
Animals were immunized with purified IgA1,k.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with both subclasses (IgA1 and IgA2).
At the request of Dr. Kearney, the line was deposited with the ATCC in 1988 by Dr. Andrew Saxon of the University of California - Los Angeles.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG1; kappa light chain
Name of Depositor
JF Kearney
Deposited As
mouse (B cell); mouse (myeloma)
References

Kiyotaki M, et al. Monoclonal anti-Id antibodies react with varying proportions of human B lineage cells. J. Immunol. 138: 4150-4158, 1987. PubMed: 3495581

Nahm MH, et al. Identification of cross-reactive antibodies with low opsonophogocytic activity for Streptoccus pneumoniae. J. Infect. Dis. 176: 698-703, 1997. PubMed: 9291318

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